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KMID : 0363320100310020254
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Journal of Korean Oriental Internal Medicine
2010 Volume.31 No. 2 p.254 ~ p.263
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Neuroprotective Effects of Bee Venom, which Removes High Molecular Elements against MPP?-induced Human Neuroblastoma SH-SY5Y Cell Death
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Bae Kwang-Rok
Doo Ah-Reum
Kim Seung-Nam
Park Ji-Yeun
Park Hi-Joon
Lee Hye-Jung
Kwon Ki-Rok
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Abstract
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Objectives : The neuroprotective effects of bee venom (BV) have been demonstrated in many studies, but bee venom has many side effects. So we used sweet bee venom (SBV), which has high molecular elements removed to reduce the side effects. I examined the neuroprotective effect of sweet bee venom in 1-methyl-4-phenylpyridine ()-induced human neuroblastoma SH-SY5Y cells.
Methods : To observe the possible toxicity of SBV itself, SH-SY5Y cells were treated with SBV in various concentrations for 3 h and in concentrations (1 and 5mM) for 24h. To investigate the protective effect of SBV against toxicity, SH-SY5Y cells were pretreated with vehicle or nontoxic concentrations of SBV for 3h and the cells were not washed, followed by incubation with respective concentrations of SBV and 1 mM for 24h. To investigate the protective effect of SBV against toxicity, SH-SY5Y cells were pretreated with vehicle or nontoxic concentrations of SBV for 3h and the cells were not washed, followed by incubation with respective of SBV(0.5%), 1 mM , 5uM AKT inhibitor(LY984002) and 10uM ERK inhibitor(PD98059) for 24 h. The protective effect was measured by cell viability assay. To investigate the degree of apoptosis, caspase-3 enzyme activity was measured in control, , SBV+.
Results : SBV (0.5%) pretreatment protected the SH-SY5Y cells against -induced apoptotic cell death. The cell viability was higher in the SH-SY5Y cells that were pretreated with vehicle or nontoxic concentrations of SBV than those not pretreated. The caspase-3 activity was lower in the pretreated groups than these not pretreated. ERK and AKT enzymes have a role in the neuroprotective effects of the sweet bee venom.
Conclusions : The results demonstrate that SBV has a protective effect on dopaminergic neurons against toxicity. This data suggest that SBV could be a potential therapeutic tool for neurodegenerative diseases such as Parkinson’s disease(PD).
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KEYWORD
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Parkinson’s disease(PD), MPTP, MPP?, SBV, Sweet bee venom, SH-SY5Y, caspase-3, ERK, AKT, Apoptosis
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